期刊
BIOTECHNOLOGY LETTERS
卷 41, 期 10, 页码 1133-1145出版社
SPRINGER
DOI: 10.1007/s10529-019-02715-0
关键词
Acetyl-CoA carboxylase; Biofuel; Chlamydomonas reinhardtii; Fatty acids; Lipid
资金
- Natural Science Foundation of Fujian Province, China [2017J01622]
- Sugar Crop Research System [CARS-170501]
Chlamydomonas reinhardtii is a photosynthetic unicellular model algae with multiple biotechnological advantages, and its fatty acids can be used to produce biofuels. Numerous studies suggest that acetyl-coA carboxylase (ACCa) catalyzes the first committed and rate-limiting step of fatty acid biosynthesis, thereby playing a central role in oil accumulation. Here, we cloned and overexpressed ACCa in C. reinhardtii to directly evaluate its effect on fatty acid synthesis. GC-MS analysis found that the unsaturated FAs contents of the CW15-24 and CW15-85 strains were 55.45% and 56.15%, which were significantly enriched compared to the wild type CW15 (48.39%). Under the optimized conditions, the content of lipid by overexpressed the ACCa gene in the mutant CW15-85 (0.46 g/l) was 1.16-fold greater than control through optimization of N and P sources. Altogether, our data clearly demonstrate that ACCa overexpression in C. reinhardtii can directly increase the synthesis of fatty acids.
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