期刊
BIOSENSORS & BIOELECTRONICS
卷 141, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111397
关键词
Cascaded toehold-mediated strand displacement reaction; Catalytic molecule machine; Exosome detection; CD63; Click chemistry
类别
资金
- National Natural Science Foundation of China [81871449, 81671781, 31200745]
Nowadays, exosomes that carry abundant information have attracted increasing attention as potent biomarkers of liquid biopsy and ideal candidates for early diagnosis and treatment of cancers. In this work, we propose a principle-of-proof biosensing method for amplified electrochemical detection of exosomes by using HepG2-derived exosomes as models. Specifically, target exosomes are enriched on anti-CD63-functionalized immunobeads and then recognized by a DNA chain containing CD63 aptamer region, which subsequently initiates a catalytic molecule machine that relies on cascade toehold-mediated strand displacement reaction. Benefiting from high efficiency of the molecule machine, the method shows a linear range from 1 x 10(5) to 5 x 10(7) particles/mL and a detection limit of 1.72 x 10(4) particles/mL toward target exosomes, better than most existing detection methods. Moreover, the method demonstrates a high specificity even in serum samples and suggests a potential use in clinic, which may provide sufficient information for disease diagnosis, especially early detection and prognosis monitoring of tumors.
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