Strategies for enhancement of alpha-linolenic acid rich lipids in Desmodesmus sp. without compromising the biomass production

The indigenous freshwater microalga Desmodesmus sp. produces ALA rich lipids (about 23%). The phytohormones (DAH and KIN; 0.5 mg L-1) increased the biomass yield and lipid content of microalga by 1.4-1.7 fold. Mixotrophic cultivation (500 mM glucose and 100 mM sodium acetate) enhanced the biomass yield and lipid content by 1.8-2.7 fold. The sodium azide (1.0 mM) led to a 1.5 fold and 1.7 fold enhancement in the lipid content and ALA fraction of total fatty acids, respectively without affecting the biomass yield. The low temperature (5 degrees C) as the second stage of cultivation enhanced the ALA fraction of total fatty acids by 1.2-1.5 fold for untreated, phytohormone supplemented and mixotrophic cultures, without affecting the biomass yield. These cultivation strategies could, therefore, be used for enhancement of ALA rich lipids in microalgae without compromising the biomass production.