Novel antibacterial polysaccharides produced by endophyte Fusarium solani DO7

In this study, the primary antibacterial ingredients in Fusarium solani DO7 were confirmed as polysaccharides. After purification, two polysaccharides, DY1 and DY2, exhibited excellent antibacterial activity, especially to S. aureus and E. coll. Moreover, the glycosidic linkages of DY1 were composed of (1 -> )-alpha-D-Glcp, (1 -> 3)-beta-L-Rhaf, (1 -> 4)-beta-D-Xylp, (1 -> 6)-alpha-D-Glcp, (1 -> 2,6)-alpha-D-Glcp and (1 -> 2)-beta-D-Galp; while the glycosidic linkages of DY2 contained (1 -> )-beta-D-Glcp, (1 -> 2)-alpha-L-Rhaf, (1 -> 3)-alpha-L-Araf, (1 -> 4)-beta-D-Glcp, (1 -> 4,6)-beta-D-Glcp and (1 -> 3)-alpha-D-Galp. Additionally, DY1 and DY2 possessed nontoxicity to RAW 264.7 cells. Both polysaccharides could significantly promote the levels of TNF-alpha, IL-6 and NO by activating TNF-alpha, IL-6 and iNOs mRNAs expression. These results indicated that DY1 and DY2 had great potential as a food preservative and immunomodulatory agent.