期刊
BIOMEDICINE & PHARMACOTHERAPY
卷 117, 期 -, 页码 -出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2019.109200
关键词
Bee pollen; Caco-2 cells; Intestinal barrier; Antioxidant and anti-inflammatory effects; Metabolomics
资金
- Basic Scientific Research Foundation of Chinese Academy of Inspection and Quarantine [2017JK011]
- National Natural Science Foundation of China [31702287]
- Agricultural Science and Technology Innovation Program under Grant (CAAS-ASTIP-2019-IAR)
- Modern Agro-industry Technology Research System from the Ministry of Agriculture of China [CARS-44]
- China Scholarship Council [201803250886]
Bee pollen (BP) is a natural medicine from the hive with various potential health-promoting benefits, but until now there is no study to determine its protective roles in inflammatory bowel disease (IBD). The aim of this study was to reveal the in vitro gastrointestinal protective effects of BP against IBD using molecular and metabolic methods. Dextran sulfate sodium (DSS) challenged Caco-2 cell monolayers were applied to mimic intestinal epithelial cell dysfunctions and metabolic disorders. The pretreatment with BP extract rich in polyphenols ameliorated DSS-induced cell viability losses. It also exerted protective effects against intestinal barrier impairment by strengthening epithelial integrity and tight junction losses induced by DSS. BP up-regulated antioxidant (NQO1, Txnrd1, Nrf2) and down-regulated inflammatory (TNF-alpha and IL-6) mRNA expressions, in accompany with MAPK signaling inhibition. Furthermore, metabolomics analysis based on UPLC-Q-TOF/MS revealed that BP, and DSS treated Caco-2 cells have different metabolomic profiles, with significant changes on key metabolites involved in glycerophospholipid metabolism. Our results showed that BP has great therapeutic potential throughout the early stages of DSS-induced colitis.
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