期刊
BIOMATERIALS
卷 212, 期 -, 页码 55-63出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2019.05.003
关键词
Hair regenerative medicine; Dermal papilla cell; Collagen microgel; Hair follicle germ; Attraction force
资金
- ministry of education, culture, sports, science and technology (MEXT) of Japan (Kakenhi)
- Kanagawa Institute of Industrial Science and Technology
Hair regenerative medicine is a promising approach for hair loss, during which autologous follicular stem cells are transplanted into regions of hair loss to regenerate hairs. Because cells transplanted as a single cell suspension scarcely generate hairs, the engineering of three-dimensional (3D) tissues before transplantation has been explored to improve this process. Here, we propose an approach to fabricate collagen-enriched cell aggregates, named hair beads (HBs), through the spontaneous constriction of cell-encapsulated collagen drops. Mouse embryonic mesenchymal cells or human dermal papilla cells were encapsulated in 2-mu l collagen microgels, which were concentrated > 10-fold in volume during 3 days of culture. Interestingly, HB constriction was attributed to attraction forces driven by myosin II and involved the upregulation of follicular genes. Single HBs with epithelial cells seeded in U-shaped microwells formed dumbbell-like structures comprising respective aggregates (named bead-based hair follicle germs, bbHFGs), during 3 days of culture. bbHFGs efficiently generated hair follicles upon intracutaneous transplantation into the backs of nude mice. Using an automated spotter, this approach was scalable to prepare a large number of bbHFGs, which is important for clinical applications. Therefore, this could represent a robust and practical approach for the preparation of germ-like tissues for hair regenerative medicine.
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