期刊
BIOCHEMISTRY
卷 58, 期 34, 页码 3561-3565出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.biochem.9b00436
关键词
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资金
- CSIR, New Delhi
- J. C. Bose National Fellowships
- IISc
- DBT-RA Program in Biotechnology Life Science
We describe a glycoconjugation strategy in which a sugar vinyl sulfoxide, acting as Michael donor, reacts efficiently with amine nucleophiles arising from the lysine side chain in peptides and proteins, at physiological pH and temperature. The method permits glycoconjugation of the lysine residues present in lysozyme with the sugar vinyl sulfoxide. The glycoconjugation of the protein abrogates the trypsin-mediated proteolysis at the lysine sites. The modified protein catalyzes digestion of the Gram-negative Escherichia coli cell wall and retains the same antimicrobial property as the native lysozyme.
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