期刊
ARCHIVES OF VIROLOGY
卷 164, 期 12, 页码 3115-3119出版社
SPRINGER WIEN
DOI: 10.1007/s00705-019-04399-3
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资金
- National Natural Science Foundation of China [No. 51378485, No. 50978250] Funding Source: Medline
A virulent phage, named ST20, infecting Escherichia coli O165:H8 was isolated from wastewater and subjected to genomic sequencing using the Illumina HiSeq system. Genomic analysis revealed that it contains double-stranded DNA, and its complete genome consists of 44,517 nucleotides with an average GC content of 50.81%. Morphological observations showed that phage ST20 belongs to the order Caudovirales and the family Siphoviridae due to its characteristic icosahedral capsid and a long noncontractile tail. This phage was further characterized by one-step growth curve analysis and measurement of its stability at 4 degrees C. The study has implications for the development of potential biocontrol agents.
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