期刊
AQUACULTURE
卷 511, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquaculture.2019.734250
关键词
Gynogenesis; Microsatellites; Blunt snout bream; Koi carp; Topmouth culter; Allo-sperm hybrid effect; Hypoxia tolerance; Cold shock
资金
- National Natural Science Foundation of China [31730098, 31802287, 31430088, 31572349, 31872315]
- earmarked fund for China Agriculture Research System [CARS-45]
- Hunan Provincial Natural Science and Technology Major Project [2017NK1031]
- Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province [20134486]
- National Key Research and Development Program of China [2018YFD0901202]
Blunt snout bream (Megalobrama amblycephala, 2n = 48, BSB) is an important fish species with great commercial value but has a shortcoming in hypoxia tolerance. Gynogenesis is an effective way to obtain improved BSB with a higher ability to tolerate hypoxia. In this study, BSB eggs were activated by UV-irradiated sperm of koi carp (Cyprinus carpio haematopterus, 2n = 100, KOC) and topmouth culter (Erythroculter ilishaeformis, 2n = 48, TC), respectively. Following cold shock to double the chromosomes, two types of gynogenetic BSB (abbreviated as GBSB-K and GBSB-T) were obtained. Comparative analyses regarding the morphological characteristics, DNA content and chromosome number showed no significant difference among BSB, GBSB-K and GBSB-T (p > 0.05). However, the results of microsatellites (Cca15-K, Cca15-T) revealed that paternal DNA fragments were detected in GBSB-K and GBSB-T. These special DNA fragments were valuable molecular markers to distinguish gynogenetic BSB from BSB. Furthermore, the experimental results showed that GBSB-K and GBSB-T showed obviously higher hypoxia-tolerant ability than BSB (p < 0.05). Both KOC and TC were effective stimuli to induce gynogenesis of BSB and to form the hybrid effect. Factors including cold shock, barely living irradiated sperm and the allo-sperm hybrid effect potentially produce stronger gynogenetic BSB with greater hypoxia-tolerance ability. The formation of the two types of gynogenetic BSB derived from the two types of allo-sperm is of great significance in fish genetic breeding.
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