期刊
ANALYTICAL CHEMISTRY
卷 91, 期 20, 页码 13237-13243出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b03674
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资金
- National Key Research and Development Program of China [2016YFA0201301]
- National Natural Science Foundation of China [21435005, 21627808, 21721003]
- Key Research Program of Frontier Sciences, CAS [QYZDY-SSW-SLH019]
- Development Project of Science and Technology of Jilin Province [20170101195JC]
Inspired by the coreactive activity of carbon nanodots (CDs) and branched polyethylenimine (BPEI) toward electrochemiluminescence (ECL) of Ru(bpy)(3)(2+), a highly luminescent and self-enhanced ECL nanohybrid (RuBCDs) was synthesized through covalently linking BPEI-coated carbon dots (BCDs) with Tris (4,4'-dicarboxylic acid-2,2'-bipyridyl) ruthenium(II) dichloride (Ru(dcbpy)(3)(2+)). The composition and morphological characterization demonstrated that the spherical Ru-BCDs particles with 12.1 +/- 1.4 nm diameter were obtained. The enhanced ECL property of RuBCDs was proved to originate from the dual coreactive contribution of BPEI and CDs as coreactants as well as the intramolecular electron transfer process, which could shorten the electron transfer path and minimize energy loss. A carbon nitride nanosheet (CNN) was utilized to stabilize the Ru-BCDs-modified glassy carbon electrode, which greatly improved the stability of solid-state ECL. By utilizing the affinity discrepancy of the CNN to single-stranded and double-stranded nucleic acids, a label-free and signal-on ECL biosensor was constructed for the determination of microRNA-133a (miR-133a), a potential biomarker of acute myocardial infarction. The designed biosensor exhibited good performance of miR-133a detection with a detection limit of 60 fM and could be used for the detection of real human serum with satisfactory results. The self-enhanced ECL nanohybrid with distinguished ECL efficiency holds a promising prospect in biosensing and bioimaging applications.
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