Ultrasensitive Detection of Amyloid-β Using Cellular Prion Protein on the Highly Conductive Au Nanoparticles-Poly(3,4-ethylene dioxythiophene)-Poly(thiophene-3-acetic acid) Composite Electrode

A highly sensitive electrochemical impedance sensor for amyloid beta oligomer (A beta O) was fabricated using a cellular prion protein (PrPC) bioreceptor linked with poly(thiophene-3-acetic acid) transducer. An additional thin layer of poly(3,4-ethylene dioxythiophene) embedded with gold nanoparticles was employed to provide high electrical conductivity and a large surface area. The sensing performace was investigated in terms of sensitivity and detection range. The fabricated sensor exhibited extremely low detection limit at a subfemtomolar level with a wide detection range from 10(-8) to 10(4) nM and its utility was established in mice infected with Alzheimer's disease (AD). The developed A beta O sensor could be utilized for early diagnosis of AD.