4.8 Article

Transient Hybridization Directed Nanoflare for Single-Molecule miRNA Imaging

期刊

ANALYTICAL CHEMISTRY
卷 91, 期 17, 页码 11122-11128

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b01766

关键词

-

资金

  1. National Natural Science Foundation of China [31971361, 31600687]
  2. Fundamental Research Funds for the Central Universities [1206009226, 12060092151, XK1802-8]
  3. 13th Five -Year Major Projects [2018ZX09721001]

向作者/读者索取更多资源

Accurate quantifications of cellular miRNAs are important not only for accelerating them becoming reliable diagnostics biomarkers but also for deeply understanding their influence on central signaling pathways. Although single-molecule miRNA imaging permits quantifying biomolecules at the single-molecule level, it is limited by the sensitivity and specificity of hybridization-based probes. We report a miRNA single-molecule imaging method by using conjugated polymer nanoparticle (CPN) labeled short DNA probe termed as a nanoflare. The transient hybridization of the nanoflares and target miRNAs yields a featured single-molecule kinetics signal rendering high single-molecule sensitivity and specificity. miRNA can be detected with a remarkable detection limit of 1 fM without using any amplification steps. The discrimination capability of homologous miRNAs was also demonstrated. Taking advantage of the featured single-molecule signal of the nanoflare, we can directly count single miR-21 molecules in single cells by using highly inclined and laminated optical sheet (HILO) microscopy. The statistics of the counting reveals miR-21's cell-to-cell fluctuation and differential expression of tumor cells and normal cells.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据