期刊
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷 411, 期 24, 页码 6475-6485出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-019-02027-2
关键词
Wilson's disease; Copper ion; Pyrophosphate; Alkaline phosphatase; Fluorescent probe
资金
- Ministry of Science and Technology of China [2016YFA0400904]
- National Natural Science Foundation of China [21725505, 21675145]
- Science and Technology Department of Zhejiang Province [GF18H180002]
Wilson's disease (WD), which might lead to acute liver failure, is an inherited disorder characterized by accumulation of copper (Cu2+) in the brain, the liver, and other vital organs. In the clinic, decreased serum alkaline phosphatase (ALP) concentration is used for WD diagnosis. But to the best of our knowledge, using a fluorescent probe to simultaneously detect multiple factors in WD (e.g., Cu2+, pyrophosphate (PPi), and ALP) has not been reported. Herein, we rationally designed a fluorescent switch (E)-8-((4-methylbenzylidene)amino)napthalen-1-amine (L) and successfully applied it for sequential and selective detections of Cu2+, PPi, and ALP in vitro, in living cells and synovial fluid samples with Off, On, and Off fluorescence signals, respectively. Considering the obvious correlations among Cu2+, PPi, and ALP in WD, we envision that our fluorescent probe L could be applied to in vitro diagnosing WD in the near future.
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