期刊
ANALYTICA CHIMICA ACTA
卷 1089, 期 -, 页码 131-143出版社
ELSEVIER
DOI: 10.1016/j.aca.2019.08.061
关键词
V2O5 nanobelts; Nitrogen-doped carbon dots; Alkaline phosphatase; Inner filter effect; Human breast cancer cells
资金
- National Natural Science Foundation of China [21838010, 21890762, 21676270]
- National Natural Science Foundation of China-Key Projects of Shanxi Coal Based Low Carbon Joint Foundation [U1610222]
- International Science Cooperation Program of Chinese Academy of Sciences [211211KYSB]
- National Key Research and Development Plan [2016YFF0203700]
In this work, the as-prepared V2O5 nanobelts can sensitively quench the fluorescence of nitrogen-doped carbon dots (N-CDs) based on the inner filter effect (IFE). In the presence of ascorbic acid (AA), the fluorescence of N-CDs can recover through the redox reaction between V2O5 nanobelts and AA. Meanwhile, in the presence of both alkaline phosphatase (ALP) and ascorbyl-2-phosphate (AAP), the fluorescence of N-CDs can also restore since AAP can be hydrolyzed into AA by ALP. Under optimum conditions, the linear range for AA is from 0.01 to 2.5 mu M with a detection limit of 3 nM and that for ALP is from 0.1 to 30 U/L with a detection limit of 0.04 U/L (S/N = 3). Particularly, the proposed probe could be successfully used to detect AA and ALP in human serum samples. Furthermore, N-CDs can be applied in fluorescence imaging of Human breast cancer cells with satisfactory results. (C) 2019 Elsevier B.V. All rights reserved.
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