期刊
ANALYTICA CHIMICA ACTA
卷 1069, 期 -, 页码 117-125出版社
ELSEVIER
DOI: 10.1016/j.aca.2019.04.023
关键词
Sandwich-type electrochemical immunosensor; Gold nanorods; Amine modified graphene; Au@Pd urchin-shaped nanostructures; Human epididymis specific protein 4 antigen
资金
- National Natural Science Foundation of China [21405095, 21575079]
- Shandong Provincial Natural Science Foundation [ZR2018MB008, ZR2018MB012]
- Key Research and Development Program of Shandong Province [2018GSF120001]
Sensitive detection of early ovarian cancer is imminent for women's health. Human epididymis specific protein 4 antigen (HE4 Ag), as a novel tumor marker, has good specificity and sensitivity in ovarian cancer markers, especially for the detection of early ovarian cancer. In this work, a novel and ultrasensitive sandwich-type amperometric electrochemical immunosensor was constructed using amine modified graphene supported gold nanorods (Au NRs/NH2-GS) as a sensor platform and core-shell Au@Pd urchin-shaped nanostructures (Au@Pd USs) as a label of the secondary antibodies (Ab(2), Au@Pd USs-Ab(2)) to realize the quantitative determination of HE4 Ag. The Au NRs/NH2-GS were used for increasing the electrode surface area and effectively immobilizing primary antibodies (Ab(1)) due to its good water-solubility. The Au@Pd USs have special morphology with high crystal surface index and good stability, capable of loading secondary antibodies (Ab(2)) and providing a larger active site for the catalysis of hydrogen peroxide (H2O2). The proposed immunosensor displays excellent performance for HE4 Ag detection over the range from 1 pmol L-1 to 50 nmol L-1 with a detection limit of 0.33 pmol L-1 (signalto-noise ratio of 3). Moreover, the designed immunosensor exhibits excellent reproducibility, selectivity, and stability, which shows great potential in clinical diagnosis. (C) 2019 Elsevier B.V. All rights reserved.
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