期刊
ADVANCED MATERIALS
卷 31, 期 50, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.201902023
关键词
affinity chromatography; bioseparation; glycoproteomics; hybrid monoliths; monolithic materials; phosphoproteomics
类别
资金
- China State Key Basic Research Program [2017YFA0505004, 2016YFA0501402]
- Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS) [DICP TMSR201601]
- National Natural Sciences Foundation of China [21575141]
- CAS-Weigao Research & Development Program [[2017]-009]
High-performance liquid chromatography integrated with tandem mass spectrometry (HPLC-MS/MS) has become a powerful technique for proteomics research. Its performance heavily depends on the separation efficiency of HPLC, which in turn depends on the chromatographic material. As the heart of the HPLC system, the chromatographic material is required to achieve excellent column efficiency and fast analysis. Monolithic materials, fabricated as continuous supports with interconnected skeletal structure and flow-through pores, are regarded as an alternative to particle-packed columns. Such materials are featured with easy preparation, fast mass transfer, high porosity, low back pressure, and miniaturization, and are next-generation separation materials for high-throughput proteins and peptides analysis. Herein, the recent progress regarding the fabrication of various monolithic materials is reviewed. Special emphasis is placed on studies of the fabrication of monolithic capillary columns and their applications in separation of biomolecules by capillary liquid chromatography (cLC). The applications of monolithic materials in the digestion, enrichment, and separation of phosphopeptides and glycopeptides from biological samples are also considered. Finally, advances in comprehensive 2D HPLC separations using monolithic columns are also shown.
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