期刊
ACS CHEMICAL BIOLOGY
卷 14, 期 9, 页码 1866-1873出版社
AMER CHEMICAL SOC
DOI: 10.1021/acschembio.9b00548
关键词
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资金
- UC Davis Department of Chemistry
- UC Davis New Research Initiatives and Collaborative Interdisciplinary Research Grant
- NIH T32 Fellowship [T32GM113770]
- NSF XSEDE program [CHE030089]
- Department of Biochemistry & Molecular Medicine
Tryptophan, an essential amino acid, is metabolized into a variety of small molecules capable of impacting human physiology, and aberrant tryptophan metabolism has been linked to a number of diseases. There are three principal routes by which tryptophan is degraded, and thus methods for measuring metabolic flux through these pathways can be used to understand the factors that perturb tryptophan metabolism and potentially to measure disease biomarkers. Here, we describe a method utilizing 6-fluorotryptophan as a probe for detecting tryptophan metabolites in ex vivo tissue samples via F-19 nuclear magnetic resonance. As a proof of concept, we demonstrate that 6-fluorotryptophan can be used to measure changes in tryptophan metabolism resulting from antibiotic-induced changes in gut microbiota composition. Taken together, we describe a general strategy for monitoring amino acid metabolism using F-19 nuclear magnetic resonance that is operationally simple and does not require chromatographic separation of metabolites.
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