4.8 Article

DARPin_9-29-Targeted Mini Gold Nanorods Specifically Eliminate HER2-Overexpressing Cancer Cells

期刊

ACS APPLIED MATERIALS & INTERFACES
卷 11, 期 38, 页码 34645-34651

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b10441

关键词

DARPin; gold nanorods; near-infrared illumination; tumor cells; molecular dynamic simulations

资金

  1. Israel Science Foundation [1589/14]
  2. Russian Science Foundation [19-1400112]
  3. Russian Foundation for Basic Research [18-29-09023]
  4. Italian Ministero dell'Istruzione, dell'Universita e della Ricerca (MIUR) project [PRIN 2010C4R8M8_002]
  5. state assignment of fundamental scientific research for state academies of sciences for 2013-2020 (theme Physical methods in medicine and biology) [00242019-0003]
  6. Russian Science Foundation [19-14-00112] Funding Source: Russian Science Foundation

向作者/读者索取更多资源

We have demonstrated that designed ankyrin repeat protein (DARPin) _9-29, which specifically targets human epidermal growth factor receptor 2 (HER2), binds tightly to gold mini nanorods (GNRs). Molecular dynamic simulations showed that a single layer of DARPin_9-29 molecules is formed on the surface of the nanorod and that conjugation with the nanorod does not involve the protein's domain responsible for specific binding to HER2. The nanorod-DARPin (DARPin-GNR) conjugate is specifically bound (in nanomolar concentrations) to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2. Illumination by near-infrared light (850 nm) led to almost complete eradication of the conjugate-treated SK-BR-3 cells; the viability of epithelial human breast cancer cells expressing normal amounts of the receptor was much less affected by the illumination. The results reported here pave the way toward application of DARPin-GNR conjugates in phototherapy of cancer.

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