期刊
ACS APPLIED MATERIALS & INTERFACES
卷 11, 期 36, 页码 32605-32612出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b08522
关键词
precise synthesis; fluorescence imaging; hepatotoxicity assessment; GSH; BODIPY; photo-induced electron transfer
资金
- National Natural Science Foundation of China [21572177, 21673173, 21807087]
- China Postdoctoral Science Foundation [2017M623225]
- Key Research and Development Program of Shaanxi [2019KWZ-07]
- Key Science and Technology Innovation Team of Shaanxi Province [2017KCT-37]
- Natural Science Basic Research Plan in Shaanxi Province of China [2016JZ004, 2018JQ3038]
- Xi'an City Science and Technology Project [2017085CG/RC048(XBDX004)]
Drug-induced hepatotoxicity is the main cause of acute liver injury, and its early diagnosis is indispensable in pharmacological and pathological studies. As a hepatotoxicity indicator, the GSH distribution in the liver could reflect the damage degree in situ. In this work, we have provided a theoretical design strategy to determine the generation of photo-induced electron transfer mechanism and achieve high selectivity for the target. After that, we precisely synthesized a novel near-infrared fluorescent probe BSR1 to specifically monitor endogenous GSH and hepatotoxicity in biosystem with a moderate fluorescent quantum yield (Phi = 0.394) and low detection limit (83 nM) under this strategy. Moreover, this mapping method for imaging GSH depletion in vivo to assay hepatotoxicity may provide a powerful molecular tool for early diagnosis of some diseases and contribute to assay hepatotoxicity for the development of new drugs. Importantly, this theoretical calculation-guided design strategy may provide an effective way for the precise synthesis of the target-specific fluorescent probe and change this research area from trial-and-error to concrete molecular engineering.
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