期刊
METABOLITES
卷 9, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/metabo9040080
关键词
surfactant lipidomics; high-resolution mass spectrometry; acute lung injury; bronchoalveolar lavage fluid; lipopolysaccharide
资金
- National Natural Science Foundation of China [81774156]
- Natural Science Foundation of Jiangsu Province [BK20161573]
- Major Research Plan of Natural Science Foundation of the Higher Education Institutions of Jiangsu Province [16KJA360002, 17KJB360007]
- Jiangsu Provincial 333 High Levels Talents Cultivation Project [BRA2016427]
- Jiangsu Provincial Six Talent Peaks Project [YY-022]
- Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Surfactant lipid metabolism is closely related to pulmonary diseases. Lipid metabolism disorder can cause lung diseases, vice versa. With this rationale, a useful method was established in this study to determine the lipidome in bronchoalveolar lavage fluid (BALF) of mice. The lipid components in BALF were extracted by liquid-liquid extraction (methanol and methyl tert-butyl ether, and water). Ultra-high-performance liquid chromatography coupled to hybrid Quadrupole-Exactive Orbitrap mass spectrometry was used to analyze the extracted samples, which showed a broad scanning range of 215-1800 m/z. With MS-DIAL software and built-in LipidBlast database, we identified 38 lipids in positive, and 31 lipids in negative, ion mode, including lysophosphatidylcholine (lysoPC), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), etc. Then, the changes of lipids in BALF of mice with acute lung injury (ALI) induced by lipopolysaccharide (LPS) was investigated, which may contribute to further exploration of the pathogenesis of ALI.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据