期刊
ANNALS OF TRANSLATIONAL MEDICINE
卷 7, 期 11, 页码 -出版社
AME PUBL CO
DOI: 10.21037/atm.2019.04.61
关键词
Pancreatic cancer; miR-19b-3p; phosphatase and tension homolog (PTEN); proliferation
资金
- National Natural Science Foundation of China [81820108006, 81670571, 81370559]
- Joint Projects in Major Diseases funding from Shanghai Municipal Commission of Health and Family Planning [2014ZYJB0201]
- Joint Projects for Novel Frontier Technology in Shanghai Municipal Hospital from Shanghai Municipal Commission of Health and Family Planning [SHDC12014122]
- Shanghai Innovation Program [12431901002]
Background: Pancreatic cancer is a common cancer with a poor prognosis and an increasing morbidity. miR-19b-3p has been implicated in some cancers, however, its role in pancreatic cancer is unclear. Methods: Human pancreatic cancer cell line Capan-2 cells were transfected with miR-19b-3p mimic and inhibitor. Cell proliferation was measured by 5-Ethynyl-2'-deoxyuridine (EdU) staining assays. Cell cycle of Capan-2 cells was examined by flow cytometry. The expression of phosphatase and tension homolog (PTEN) was determined by real-time quantitative polymerase chain reaction (PCR) and western blotting analysis. Functional rescue experiments were performed through PTEN overexpression and miR-19b-3p mimic by using EdU staining assays. Results: miR-19b-3p mimic significantly increased miR-19b-3p while miR-19b-3p inhibitor decreased that. EdU staining showed that miR-19b-3p overexpression promoted Capan-2 cells proliferation while miR-19b-3p inhibition decreased that. Flow cytometry analysis of cell cycle indicated that miR-19b-3p overexpression increased the percentage of Capan-2 cells in S phase while miR-19b-3p inhibition decreased that. PTEN was confirmed to be a target gene of miR-19b-3p and PTEN overexpression eliminated the pro-proliferation effects of miR-19b-3p in Capan-2 cells. Conclusions: Our study demonstrates that miR-19b-3p promotes Capan-2 cells proliferation by targeting PTEN.
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