期刊
SCIENCE IMMUNOLOGY
卷 4, 期 35, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciimmunol.aaw0902
关键词
-
类别
资金
- Medical Research Council
- Diabetes UK
- Rosetrees Trust
- European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant [675395]
- Wellcome Trust
- MRC [MR/N001435/1] Funding Source: UKRI
CTLA-4 is a critical negative regulator of the immune system and a major target for immunotherapy. However, precisely how it functions in vivo to maintain immune homeostasis is not clear. As a highly endocytic molecule, CTLA-4 can capture costimulatory ligands from opposing cells by a process of transendocytosis (TE). By restricting costimulatory ligand expression in this manner, CTLA-4 controls the CD28-dependent activation of T cells. Regulatory T cells (T-regs) constitutively express CTLA-4 at high levels and, in its absence, show defects in TE and suppressive function. Activated conventional T cells (T-conv) are also capable of CTLA-4-dependent TE; however, the relative use of this mechanism by T-regs and T-conv in vivo remains unclear. Here, we set out to characterize both the perpetrators and cellular targets of CTLA-4 TE in vivo. We found that T-regs showed constitutive cell surface recruitment of CTLA-4 ex vivo and performed TE rapidly after TCR stimulation. T-regs outperformed activated T-conv at TE in vivo, and expression of ICOS marked T-regs with this capability. Using TCR transgenic T-regs that recognize a protein expressed in the pancreas, we showed that the presentation of tissue-derived self-antigen could trigger T-regs to capture costimulatory ligands in vivo. Last, we identified migratory dendritic cells (DCs) as the major target for T-reg-based CTLA-4-dependent regulation in the steady state. These data support a model in which CTLA-4 expressed on T-regs dynamically regulates the phenotype of DCs trafficking to lymph nodes from peripheral tissues in an antigen-dependent manner.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据