期刊
ARCHIVES OF VIROLOGY
卷 161, 期 3, 页码 749-753出版社
SPRINGER WIEN
DOI: 10.1007/s00705-015-2737-z
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资金
- Young Talents Training Program of Shandong Academy of Agricultural Sciences
- International Science and Technology Cooperation Program of China [2012DFR30700]
- Special Fund for Agro-Scientific Research in the Public Interest [201203075]
- National Key Technology Research and Development Program of the Ministry of Science and Technology of China [2013BAD02B03-3-2]
- Special Fund for Innovation Teams of Fruit Trees in Agricultural Technology System of Shandong Province [SDAIT-03-022-04]
Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from Taian, China (LChV-1-TA), detected by small-RNA deep sequencing and amplified by overlapping RT-PCR. The LChV-1-TA genome was 16,932 nt in length and contained nine open reading frames (ORFs), with sequence identity at the overall genome level of 76 %, 76 %, and 78 % to LChV-1 isolates Y10237 (UW2 isolate), EU715989 (ITMAR isolate) and JX669615 (V2356 isolate), respectively. Based on the phylogenetic analysis of HSP70h amino acid sequences of Closteroviridae family members, LChV-1-TA was grouped into a well-supported cluster with the members of the genus Velarivirus and was also closely related to other LChV-1 isolates. This is the first report of the complete nucleotide sequence of LChV-1 infecting sweet cherry in China.
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