期刊
CELLS
卷 8, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/cells8070707
关键词
fungal biofilm; oral epithelium; apoptosis; beta-defensin; epithelial defense
类别
资金
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil) [2011/17481-6, 2015/03965-2]
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil) [307603/2018-0]
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brazil) [001]
The presence of Candida albicans in the biofilm underlying the dental prosthesis is related to denture stomatitis (DS), an inflammatory reaction of the oral mucosa. The oral epithelium, a component of the innate immune response, has the ability to react to fungal invasion. In this study, we evaluated the in vitro effect of viable C. albicans on the apoptosis, nitric oxide (NO) production, and beta-defensin 2 (hBD-2) expression and production of human palate epithelial cells (HPECs). We further determined whether or not these effects were correlated with fungal invasion of epithelial cells. Interaction between HPEC primary culture and C. albicans was obtained through either direct or indirect cell cell contact with a supernatant from a hyphal fungus. We found that the hyphae supernatants were sufficient to induce slight HPEC apoptosis, which occurred prior to the activation of the specific mechanisms of epithelial defense. The epithelial defense responses were found to occur via NO and antimicrobial peptide hBD-2 production only during direct contact between C. albicans and HPECs and coincided with the fungus's intraepithelial invasion. However, although the hBD-2 levels remained constant in the HPEC supernatants over time, the NO release and hBD-2 gene expression were reduced at a later time (10 h), indicating that the epithelial defense capacity against the fungal invasion was not maintained in later phases. This aspect of the immune response was associated with increased epithelial invasion and apoptosis maintenance.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据