期刊
ARCHIVES OF VIROLOGY
卷 162, 期 2, 页码 495-500出版社
SPRINGER WIEN
DOI: 10.1007/s00705-016-3116-0
关键词
Beet necrotic yellow vein virus (BNYVV); DAS-ELISA; IC-RT-LAMP; RT-LAMP; Sugar beet
类别
资金
- Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran
Sugar beet can be infected by many different viruses that can reduce yield; beet necrotic yellow vein virus (BNYVV) is one of the most economically important viruses of this crop plant. This report describes a new reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for identification of BNYVV. In addition, a novel immunocapture (IC) RT-LAMP assay for rapid and easy detection (without RNA extraction) of BNYVV was developed here and compared with DAS-ELISA and RT-LAMP assays. Our results show that the IC-RT-LAMP assay is a highly reliable alternative assay for identification of BNYVV.
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