期刊
FRONTIERS IN CHEMISTRY
卷 7, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fchem.2019.00435
关键词
rice false smut disease; Ustilaginoidea virens; sorbicillinoids; phytotoxic activity; cytotoxic activity
资金
- National Key R&D Program of China [2017YFC1600905, 2017YFD0201105]
- National Natural Science Foundation of China [31471729, 31271996]
Ustilaginoidea virens, the causal fungus of rice false smut, was found in previous studies to produce two types of metabolites, ustiloxins and ustilaginoidins. However, genome sequencing of U. virens revealed a plethora of secondary-metabolites-biosynthetic core genes that were capable to biosynthesize unreported metabolites. A large-scale fermentation of U. virens was thus performed, and the fungal extract was chemically re-investigated. After removing the known metabolites, we found a fraction containing unknown phytotoxic substances. Fractionation of this part has led to the isolation of six new sorbicillinoids, namely ustisorbicillinols A similar to F (1 similar to 6), and two new sorbicillinoid-related pyrones, named ustilopyrones A (7) and B (8), together with nine known cogeners (9 similar to 17). The structures of the new compounds were elucidated by analysis of their NMR, HRMS, and CD spectra, while ECD, C-13 NMR and optical rotation calculations were additionally used for configurational assignments. Plausible biosynthetic pathways for the new compounds were proposed. Phytotoxicity assays revealed that the major sorbicillinoids (12 similar to 14, and 16) showed strong inhibition against the radicle and germ elongation of rice and lettuce seeds, with compound 12 displaying the strongest inhibition. The isolated compounds were also evaluated for their cytotoxic, antibacterial, and antifungal activities. Compounds 10, and 12 similar to 14 showed moderate cytotoxicities against the tested cell lines with IC(50)s of 8.83 similar to 74.7 mu M. Compounds 2, and 10 similar to 13 were active against the tested bacteria (MICs 4 similar to 128 mu g/mL), while compounds 11 similar to 13 displayed moderate antifungal activities.
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