4.8 Article

NF-κB Signaling and IL-4 Signaling Regulate SATB1 Expression via Alternative Promoter Usage During Th2 Differentiation

期刊

FRONTIERS IN IMMUNOLOGY
卷 10, 期 -, 页码 -

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2019.00667

关键词

SATB1; alternative promoter; TCR signaling; cytokine signaling; STAT6

资金

  1. Center of Excellence in Epigenetics program of the Department of Biotechnology [BT/01/COE/09/07, BT/MED/30/SP11288/2015]
  2. IISER Pune
  3. Academy of Finland, AoF, Center of Excellence in Molecular Systems Immunology and Physiology Research [250114]
  4. AoF [292335, 294337, 292482, 298732, 298998, 315585]
  5. Sigrid Juselius Foundation (SJF)
  6. IISER-Pune
  7. DST-SERB (N-PDF)
  8. DBT (RA)
  9. Academy of Finland [258313]
  10. Academy of Finland (AKA) [258313, 258313] Funding Source: Academy of Finland (AKA)

向作者/读者索取更多资源

SATB1 is a genome organizer protein that is expressed in a lineage specific manner in CD4(+) T-cells. SATB1 plays a crucial role in expression of multiple genes throughout the thymic development and peripheral differentiation of T cells. Although SATB1 function has been subjected to intense investigation, regulation of SATB1 gene expression remains poorly understood. Analysis of RNA-seq data revealed multiple transcription start sites at the upstream regulatory region of SATB1. We further demonstrated that SATB1 gene is expressed via alternative promoters during T-helper (Th) cell differentiation. The proximal promoter P1 is used more by the naive and activated CD4(+) T-cells whereas the middle P2 and the distal P3 promoters are used at a significantly higher level by polarized T-helper cells. Cytokine and TCR signaling play crucial roles toward SATB1 alternative promoter usage. Under Th2 polarization conditions, transcription factor STAT6, which operates downstream of the cytokine signaling binds to the P2 and P3 promoters. Genetic perturbation by knockout and chemical inhibition of STAT6 activation resulted in the loss of P2 and P3 promoter activity. Moreover, chemical inhibition of activation of NF-KB, a transcription factor that operates downstream of the TCR signaling, also resulted in reduced P2 and P3 promoter usage. Furthermore, usage of the P1 promoter correlated with lower SATB1 protein expression whereas P2 and P3 promoter usage correlated with higher SATB1 protein expression. Thus, the promoter switch might play a crucial role in fine-tuning of SATB1 protein expression in a cell type specific manner.

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