期刊
FRONTIERS IN IMMUNOLOGY
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2019.01223
关键词
chronic kidney disease; fibrosis; NLRP3 inflammasome; uremia; diabetes
类别
资金
- Hoffmann La Roche
- Deutsche Forschungsgemeinschaft [AN372/24-1, AN372/27-1]
- Chinese Scholarship Council
- Else Kroner-Fresenius Foundation
Inflammasome-driven release of interleukin(IL)-1 beta is a central element of many forms of sterile inflammation and has been evident to promote the onset and progression of diabetic kidney disease. We microdissected glomerular and tubulointerstitial samples from kidney biopsies of patients with diabetic kidney disease and found expression of IL-1 beta mRNA. Immunostaining of such kidney biopsies across a broad spectrum of diabetic kidney disease stages revealed IL-beta positivity in a small subset of infiltrating immune cell. Thus, we speculated on a potential of IL-1 beta as a therapeutic target and neutralizing the biological effects of murine IL-1 beta with a novel monoclonal antibody in uninephrectomized diabetic db/db mice with progressive type 2 diabetes- and obesity-related single nephron hyperfiltration, podocyte loss, proteinuria, and progressive decline of total glomerular filtration rate (GFR). At 18 weeks albuminuric mice were randomized to intraperitoneal injections with either anti-IL-1 beta or control IgG once weekly for 8 weeks. During this period, anti-IL-1 beta IgG had no effect on food or fluid intake, body weight, and fasting glucose levels. At week 26, anti-IL-1 beta IgG had reduced renal mRNA expression of kidney injury markers (Ngal) and fibrosis (Col1, a-Sma), significantly attenuated the progressive decline of GFR in hyperfiltrating diabetic mice, and preserved podocyte number without affecting albuminuria or indicators of single nephron hyperfiltration. No adverse effect were observed. Thus, IL-1 beta contributes to the progression of chronic kidney disease in type 2 diabetes and might therefore be a valuable therapeutic target, potentially in combination with drugs with different mechanisms-of-action such as RAS and SGLT2 inhibitors.
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