期刊
ARCHIVES OF PHARMACAL RESEARCH
卷 40, 期 1, 页码 37-48出版社
PHARMACEUTICAL SOC KOREA
DOI: 10.1007/s12272-016-0852-3
关键词
Prunin; Protein tyrosine phosphatase 1B; Anti-diabetic; Glucose uptake; HepG2 cell; Molecular docking
Prunin is the main flavonoid in Prunus davidiana stems and improves hyperglycemia and hyperlipidemia in streptozotocin-induced diabetic rats. The aim of this study was to investigate the in vitro anti-diabetic potential of prunin via the inhibition of protein tyrosine phosphatase 1B (PTP1B), alpha-glucosidase, peroxynitrite (ONOO-)-mediated tyrosine nitration, and stimulation of glucose uptake in insulin-resistant hepatocytes. In addition, a molecular docking simulation was performed to predict specific prunin binding modes during PTP1B inhibition. Prunin showed strong inhibitory activity against PTP1B, with an IC50 value of 5.5 +/- 0.29 A mu M, and significant inhibitory activity against alpha-glucosidase, with an IC50 value of 317 +/- 2.12 A mu M. Moreover, a kinetics study revealed that prunin inhibited PTP1B (K (i) = 8.66) and alpha-glucosidase (K (i) = 189.56) with characteristics typical of competitive and mixed type inhibitors, respectively. Docking simulations showed that prunin selectively inhibited PTP1B by targeting its active site and exhibited good binding affinity, with a docking score of -9 kcal/mol. Furthermore, prunin exhibited dose-dependent inhibitory activity against ONOO--mediated tyrosine nitration and stimulated glucose uptake by decreasing PTP1B expression level in insulin-resistant HepG2 cells. These results indicate that prunin has significant potential as a selective PTP1B inhibitor and may possess anti-diabetic properties by improving insulin resistance.
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