期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 149, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/59559
关键词
Developmental Biology; Issue 149; skeletal muscle; X-ray diffraction; acto-myosin interaction; sarcomere structure; skeletal muscle myopathy; skeletal muscle physiology
资金
- DOE Office of Science [DE-AC02-06CH11357]
- National Institute of General Medical Sciences of the National Institutes of Health [P41 GM103622]
- NIGMS [1S10OD018090-01]
Transgenic mouse models have been important tools for studying the relationship of genotype to phenotype for human diseases including those of skeletal muscle. Mouse skeletal muscle has been shown to produce high quality X-ray diffraction patterns on third generation synchrotron beamlines providing an opportunity to link changes at the level of the genotype to functional phenotypes in health and disease by determining the structural consequences of genetic changes. We present detailed protocols for preparation of specimens, collecting the X-ray patterns and extracting relevant structural parameters from the X-ray patterns that may help guide experimenters wishing to perform such experiments for themselves.
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