期刊
FRONTIERS IN PHARMACOLOGY
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2019.00811
关键词
Cyclocarya paliurus; triterpene; diabetes mellitus; hepatic inflammation; ROCK; NF-kappa B
资金
- National Natural Science Foundation of China [81503316, 81872984]
- Natural Science Foundation of Jiangsu Province [BK20161460]
- Traditional Chinese Medicine Science and Technology Project of Jiangsu Province [YB2017031, YB2017028]
This study aimed to assess the effects of triterpene extract of Cyclocarya paliurus (Batal.) Iljinskaja (CPT) on diabetes-induced hepatic inflammation and to unveil the underlying mechanisms. Diabetes in db/db mice was alleviated after CPT administration, as assessed by the oral glucose tolerance test. In addition, treatment with CPT dramatically reduced serum insulin, aspartate amino-transaminase, alanine aminotransferase, triglyceride, and total cholesterol amounts. Besides, serum levels of interleukin (IL)-1 beta, IL-6, and tumor necrosis factor (TNF)-alpha were also reduced after CPT administration. Western blot analysis revealed that CPT treatment significantly reversed the protein expression levels of Rho, ROCK1, ROCK2, p-P65, p-I kappa B alpha, p-IKK alpha, and p-IKK beta in liver samples obtained from db/db mice. Upon palmitic acid stimulation, the protective effects of CPT on the liver were further assessed in HepG2 and LO2 cells, and no appreciable cytotoxic effects were found. Therefore, these findings indicate that CPT alleviates liver inflammation via Rhokinase signaling.
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