期刊
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
卷 47, 期 1, 页码 2230-2239出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/21691401.2019.1622552
关键词
Chamomile; manganese dioxide nanoparticles; magnesium oxide nanoparticles; Acidovorax oryzae; flow cytometry; antibacterial activity
资金
- National Natural Science Foundation of China [31872017, 31571971, 31371904, 31801787]
- Zhejiang Provincial Natural Science Foundation of China [LZ19C140002]
- Zhejiang Provincial Project [2017C02002, 2019C02006]
- National Key Research and Development Program of China [2017YFD0201104]
- Shanghai Agricultural Basic Research Project [2014:7-3-1]
- Key Scientific Technological Project of Ningbo [2016C11017]
- Fundamental Research Funds for the Central Universities
- Dabeinong Funds for Discipline Development and Talent Training in Zhejiang University
- Key Subject Construction Program of Zhejiang for Modern Agricultural Biotechnology and Crop Disease Control, China Postdoctoral Science Special Foundation [517000-X91803]
- Zhejiang Provincial Postdoctoral Foundation [517000-X81802]
Bacterial brown stripe (BBS) is one of the most economically important diseases of rice caused by Acidovorax oryzae (Ao). In order to ensure food security and safe consumption, the use of non-chemical approach is necessary. In this study, MgO and MnO2 were synthesized using chamomile flower extract. The synthesized MgO and MnO2 nanoparticles were characterized by UV-Visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission/scanning electron microscopy. The sizes were 18.2 and 16.5 nm for MgO and MnO2 nanoparticles, respectively. The MgO and MnO2 nanoparticles reduced the growth of Ao strain RS-2 by 62.9 and 71.3%, respectively. Also, the biofilm formation and swimming motility were significantly reduced compared to the control. The antibacterial mechanisms of MgO and MnO2 nanoparticles against RS-2 reveals that MgO and MnO2 nanoparticles penetrated the cells and destroyed the cell membrane leading to leakage of cytoplasmic content. Also, the flow cytometry observation reveals that the apoptotic cell ratio of RS-2 increased from 0.97% to 99.52 and 99.94% when treated with MgO and MnO2 nanoparticles, respectively. Altogether, the results suggest that the synthesized MgO and MnO2 nanoparticles could serve as an alternative approach method for the management of BBS.
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