期刊
ARCHIVES OF MICROBIOLOGY
卷 198, 期 7, 页码 603-610出版社
SPRINGER
DOI: 10.1007/s00203-016-1219-3
关键词
E. coli strain Nissle 1917; Flagella display application; Flagellin
类别
资金
- Chinese National Science Foundation [30571374, 30771603, 31072136, 31270171]
- Genetically Modified Organisms Technology Major Project of China [2014ZX08006-001B]
The most studied probiotic, Escherichia coli strain Nissle 1917 (EcN) possesses flagella of serotype H1. To explore the potential to use EcN flagellin in flagella display applications, we investigated the effect of deleting amino acids in the hypervariable region of flagellin on EcNc (EcN cured of its two cryptic plasmids pMUT1 and pMUT2). Two EcNc flagellin isogenic mutants with deletions of amino acid residual from 277 to 286 and from 287 to 296 in the hypervariable domain were constructed. Both mutants were flagellated, adherent to IPEC-J2 cells, and colonized BALB/c mice. These hypervariable regions may have future utility in the display of heterologous epitopes.
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