期刊
TOXINS
卷 11, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/toxins11070388
关键词
cyanobacterial toxin; deuterated MC standards; microcystin; blood; liver tissue; UPLC-MS/MS; quantification
资金
- Arthur-and-Aenne-Feindt Foundation (Hamburg Germany)
- Marie Curie International Research Sta ff Exchange Scheme Fellowship [PIRSES-GA-2011-295223]
- Royal Society of New Zealand International Research Sta ff Exchange Scheme Fellowship [295223]
- Marsden Fund of the Royal Society of New Zealand [CAW1601]
- Konstanz Research School Chemical Biology (KoRS-CB)
- CHARM (Baden-Wurttemberg Wassernetzwerk)
- DFG [INST 38-537-1]
Cyanobacterial microcystins (MCs), potent serine /threonine-phosphatase inhibitors, pose an increasing threat to humans. Current detection methods are optimised for water matrices with only a few MC congeners simultaneously detected. However, as MC congeners are known to di ff er in their toxicity, methods are needed that simultaneously quantify the congeners present, thus allowing for summary hazard and risk assessment. Moreover, detection of MCs should be expanded to complex matrices, e. g., blood and tissue samples, to verify in situ MC concentrations, thus providing for improved exposure assessment and hazard interpretation. To achieve this, we applied two synthetic deuterated MC standards and optimised the tissue extraction protocol for the simultaneous detection of 14 MC congeners in a single ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS /MS) run. This procedure was validated using plasma and liver homogenates of mice (male and female) spiked with deuterated MC standards. For proof of concept, tissue and plasma samples from mice i. p. injected with MC-LR and MC-LF were analysed. While MC-LF was detected in all tissue samples of both sexes, detection of MC-LR was restricted to liver samples of male mice, suggesting di ff erent toxicokinetics in males, e. g., transport, conjugation or protein binding. Thus, deconjugation /-proteinisation steps should be employed to improve detection of bound MC.
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