期刊
CELL REPORTS
卷 28, 期 5, 页码 1206-+出版社
CELL PRESS
DOI: 10.1016/j.celrep.2019.06.097
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资金
- Canadian Institutes of Health Research [CIHR MOP-133648, MOP-162334]
- Fonds de Recherche du Quebec - Nature et Technologies (FRQNT)
- Calcul Quebec
- Compute Canada
Genomic DNA is framed by additional layers of information, referred to as the epigenome. Epigenomic marks such as DNA methylation, histone modifications, and histone variants are concentrated on specific genomic sites, where they can both instruct and reflect gene expression. How this information is maintained, notably in the face of transcription, is not completely understood. Specifically, the extent to which modified histones themselves are retained through RNA polymerase II passage is unclear. Here, we show that several histone modifications are mislocalized when the transcription-coupled histone chaperones FACT or Spt6 are disrupted in Saccharomyces cerevisiae. In the absence of functional FACT or Spt6, transcription generates nucleosome loss, which is partially compensated for by the increased activity of non-transcription-coupled histone chaperones. The random incorporation of transcription-evicted modified histones scrambles epigenomic information. Our work highlights the importance of local recycling of modified histones by FACT and Spt6 during transcription in the maintenance of the epigenomic landscape.
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