期刊
CELL REPORTS
卷 28, 期 1, 页码 11-+出版社
CELL PRESS
DOI: 10.1016/j.celrep.2019.06.005
关键词
-
类别
资金
- EU FP7 Marie Curie Integration Grant [PCIG11-GA-2012-321905]
- Deutsche Forschungsgemeinschaft [FOR 2419 WA3716/1-1, FOR 2419 HE8413/1-1, FOR 2419 KN556/11-1, KN556/11-2]
- Hamburg Landesforschungsforderung grant [LFF-FV27]
- Ritz-Stiftung
Myosin VI is an actin-based cytoskeletal motor implicated in various steps of membrane trafficking. Here, we investigated whether this myosin is crucial for synaptic function and plasticity in neurons. We find that myosin VI localizes at cerebellar parallel fiber to Purkinje cell synapses and that the myosin is indispensable for long-term depression of AMPA-receptor-mediated synaptic signal transmission at this synapse. Moreover, direct visualization of GluA2-containing AMPA receptors in Purkinje cells reveals that the myosin drives removal of AMPA receptors from the surface of dendritic spines in an activity-dependent manner. Co-immunoprecipitation and super-resolution microscopy indicate that specifically the interaction of myosin VI with the clathrin adaptor component alpha-adaptin is important during long-term depression. Together, these data suggest that myosin VI directly promotes clathrin-mediated endocytosis of AMPA receptors in Purkinje cells to mediate cerebellar long-term depression. Our results provide insights into myosin VI function and the molecular mechanisms underlying synaptic plasticity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据