期刊
CELL REPORTS
卷 27, 期 10, 页码 2990-+出版社
CELL PRESS
DOI: 10.1016/j.celrep.2019.05.003
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资金
- Royal Society [UF100717, UF150651]
- Fell Fund [153/092]
- OCRC/Cancer Research UK (CR-UK) [OCRC 0213-MC, CRUKDF 0715-MC]
- Medical Research Fund
- Medical Research Council (MRC) [MR/N021002/1]
- Wellcome Trust Senior Research Fellowship [210640/Z/18/Z]
- MRC-Clarendon Scholarship
- Mutua Madrilena'' Graduate Scholarship
- Taiwanese Government
- Goodger Scholarship
- Postdoctoral Fellowship from the Japan Society for the Promotion of Science (JSPS)
- Royal Society [UF100717] Funding Source: Royal Society
- MRC [MR/N021002/1] Funding Source: UKRI
- Wellcome Trust [210640/Z/18/Z] Funding Source: Wellcome Trust
Interstrand crosslinks (ICLs) of the DNA helix are a deleterious form of DNA damage. ICLs can be repaired by the Fanconi anemia pathway. At the center of the pathway is the FANCD2/FANCI complex, recruitment of which to DNA is a critical step for repair. After recruitment, monoubiquitination of both FANCD2 and FANCI leads to their retention on chromatin, ensuring subsequent repair. However, regulation of recruitment is poorly understood. Here, we report a cluster of phosphosites on FANCD2 whose phosphorylation by CK2 inhibits both FANCD2 recruitment to ICLs and its monoubiquitination in vitro and in vivo. We have found that phosphorylated FANCD2 possesses reduced DNA binding activity, explaining the previous observations. Thus, we describe a regulatory mechanism operating as a molecular switch, where in the absence of DNA damage, the FANCD2/FANCI complex is prevented from loading onto DNA, effectively suppressing the FA pathway.
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