期刊
CELL REPORTS
卷 27, 期 13, 页码 3916-+出版社
CELL PRESS
DOI: 10.1016/j.celrep.2019.05.084
关键词
-
类别
资金
- NIH [R01CA125577, R01CA107469, F30CA19084, P30CA046592]
- Forbes Institute for Cancer Discovery
- Cancer Center Shared Resources: Flow Cytometry, University of Michigan Office of Research (UMOR) [26998]
- Cancer Center Shared Resources: Cell & Tissue Imaging, University of Michigan Office of Research (UMOR) [26998]
- Cancer Center Shared Resources: Tissue & Molecular Pathology, University of Michigan Office of Research (UMOR) [26998]
Twenty percent of breast cancer (BC) patients develop distant metastasis for which there is no cure. Mesenchymal stem/stromal cells (MSCs) in the tumor microenvironment were shown to stimulate metastasis, but the mechanisms are unclear. Here, we identified and quantified cancer cells engulfing stromal cells in clinical samples of BC metastasis by dual immunostaining for EZH2 and ALDH1 expression. Using flow cytometry and a microfluidic single-cell paring and retrieval platform, we show that MSC engulfment capacity is associated with BC cell metastatic potential and generates cells with mesenchymal-like, invasion, and stem cell traits. Whole-transcriptome analyses of selectively retrieved engulfing BC cells identify a gene signature of MSC engulfment consisting of WNT5A, MSR1, ELMO1, IL1RL2,ZPLD1, and SIRPB1. These results delineate a mechanism by which MSCs in the tumor microenvironment promote metastasis and provide a microfluidic platform with the potential to predict BC metastasis in clinical samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据