期刊
ACS SYNTHETIC BIOLOGY
卷 8, 期 8, 页码 1867-1876出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.9b00187
关键词
protein scaffolds; immobilization; synthetic biology; biocatalysis; alcohol dehydrogenase; enzyme
资金
- Defense Threat Reduction Agency [HDTRA1-15-0004]
- Defense Advanced Research Projects Agency [HR0011-17-2-0038]
- University of Minnesota
Immobilization of enzymes is required for most biocatalytic processes, but chemistries used in enzyme immobilization are limited and can be challenging. Genetically encoded protein-based biomaterials could provide easy-to-use immobilization platforms for biocatalysts. We recently developed a self-assembling protein scaffold that covalently immobilized SpyTagged enzymes by engineering the bacterial microcompartment protein EutM from Salmonella enterica with a SpyCatcher domain. We also identified a range of EutM homologues as robust protein nanostructures with diverse architectures and electrostatic surface properties. In this work, we created a modular immobilization platform with tunable surface properties by developing a toolbox of self-assembling, robust EutM-SpyCatcher scaffolds. Using an alcohol dehydrogenase as model biocatalyst, we show that the scaffolds improve enzyme activity and stability. This work provides a modular, easy-to-use immobilization system that can be tailored for the optimal function of biocatalysts of interest.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据