期刊
BIOMICROFLUIDICS
卷 9, 期 1, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.4907896
关键词
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资金
- European Commission (Project PASCA) [FP7 GA257073]
Label-free isolation of single cells is essential for the growing field of single-cell analysis. Here, we present a device which prints single living cells encapsulated in free-flying picoliter droplets. It combines inkjet printing and impedance flow cytometry. Droplet volume can be controlled in the range of 500 pl-800 pl by piezo actuator displacement. Two sets of parallel facing electrodes in a 50 mu m x 55 mu m channel are applied to measure the presence and velocity of a single cell in real-time. Polystyrene beads with < 5% variation in diameter generated signal variations of 12%-17% coefficients of variation. Single bead efficiency (i.e., printing events with single beads vs. total number of printing events) was 73% +/- 11% at a throughput of approximately 9 events/min. Viability of printed HeLa cells and human primary fibroblasts was demonstrated by culturing cells for at least eight days. (c) 2015 AIP Publishing LLC.
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