Fabrication of laser printed microfluidic paper-based analytical devices (LP-μPADs) for point-of-care applications

Microfluidic paper-based analytical devices (mu PADs) have provided a breakthrough in portable and low-cost point-of-care diagnostics. Despite their significant scope, the complexity of fabrication and reliance on expensive and sophisticated tools, have limited their outreach and possibility of commercialization. Herein, we report for the first time, a facile method to fabricate mu PADs using a commonly available laser printer which drastically reduces the cost and complexity of fabrication. Toner ink is used to pattern the mu PADs by printing, without modifying any factory configuration of the laser printer. Hydrophobic barriers are created by heating the patterned paper which melts the toner ink, facilitating its wicking into the cross-section of the substrate. Further, we demonstrate the utilization of the fabricated device by performing two assays. The proposed technique provides a versatile platform for rapid prototyping of mu PADs with significant prospect in both developed and resource constrained region.