期刊
SCIENTIFIC REPORTS
卷 9, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-019-41788-9
关键词
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资金
- Instituto de Salud Carlos III [PI15CIII/00031]
- Spanish Ministry of Economy and Competitiveness [SAF2016-78480-R]
- SPANISH AIDS Research Network [RD16CIII/0002/0001, RD16CIII/0002/0002, RD16/0025/0013]
- ISCIII - Subdireccion General de Evaluacion and European Funding for Regional Development (FEDER) [PIE 13/00040, RD12/0017/0017]
- Portuguese Fundacao para a Ciencia e Tecnologia (FCT) (EDCTP2 programme - European Union) [SFRH/BPD/77448/2011]
- Miguel Servet programme from Fondo de Investigacion Sanitaria (ISCIII) [CP13/00098, CP14/CIII/00010, CP14/00198]
In HIV-1/HCV-coinfected patients, chronic HCV infection leads to an increased T-lymphocyte immune activation compared to HIV-monoinfected patients, thereby likely contributing to increase HIV-1 reservoir that is the major barrier for its eradication. Our objective was to evaluate the influence of HCV coinfection in HIV-1 viral reservoir size in resting (r) CD4+ T-cells (CD25-CD69-HLADR-). Multicenter cross-sectional study of 97 cART-treated HIV-1 patients, including 36 patients with HIV and HCV-chronic co-infection without anti-HCV treatment, 32 HIV patients with HCV spontaneous clearance and 29 HIV-monoinfected patients. rCD4+ T-cells were isolated and total DNA was extracted. HIV viral reservoir was measured by Alu-LTR qPCR. Differences between groups were calculated with a generalized linear model. Overall, 63.9% were men, median age of 41 years and Caucasian. Median CD4+ and CD8+ T-lymphocytes were 725 and 858 cells/mm(3), respectively. CD4+ T nadir cells was 305 cells/mm(3). Proviral HIV-1 DNA size was significantly increased in chronic HIV/HCV-coinfected compared to HIV-monoinfected patients (206.21 +/- 47.38 vs. 87.34 +/- 22.46, respectively; P = 0.009), as well as in spontaneously clarified HCV co-infected patients when compared to HIV-monoinfected individuals (136.20 +/- 33.20; P = 0.009). HIV-1/HCV co-infected patients showed a larger HIV-1 reservoir size in comparison to HIV-monoinfected individuals. This increase could lead to a greater complexity in the elimination of HIV-1 reservoir in HIV-1/HCV-coinfected individuals, which should be considered in the current strategies for the elimination of HIV-1 reservoir.
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