期刊
BIOMEDICAL OPTICS EXPRESS
卷 10, 期 8, 页码 4329-4339出版社
OPTICAL SOC AMER
DOI: 10.1364/BOE.10.004329
关键词
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资金
- National Institutes of Health (NIH) [R33CA223581, R01CA224275]
- National Science Foundation (NSF) [CHE1807106]
Thin tissue slice based histology has been used as a gold standard for disease diagnosis since over a hundred years ago. However, histopathological evaluation on two-dimensional slides suffers from large variations duc to limited sampling. To improve the diagnostic accuracy, three-dimensional (3D) histology is performed through serial sectioning, staining, imaging and reconstruction of individual slices, which is highly time-consuming and labor intensive. We developed a volumetric stimulated Raman scattering (SRS) imaging method, which provides histology-like information in 3D context without the need for staining with dyes. Using a small molecule clearing agent, formamide, we performed tissue clearing Within 30 min and achieved an imaging depth up to 500 pm in highly scattered tissues, including brain, kidney; liver and lung. Through a two-color SRS imaging scheme, we obtained histology-like images in cleared brain tissue slices. Our method has the potential for 3D tissue histopathology to improve the accuracy of histopathological examination. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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