4.6 Article

Speckle-structured illumination for 3D phase and fluorescence computational microscopy

期刊

BIOMEDICAL OPTICS EXPRESS
卷 10, 期 7, 页码 3635-3653

出版社

OPTICAL SOC AMER
DOI: 10.1364/BOE.10.003635

关键词

-

资金

  1. STROBE: A National Science Foundation Science & Technology Center [DMR 1548924]
  2. Gordon and Betty Moore Foundation's Data-Driven Discovery Initiative [GBMF4562]
  3. Ruth L. Kirschstein National Research Service Award [F32GM129966]
  4. Chan Zuckerberg Biohub

向作者/读者索取更多资源

High-content biological microscopy targets high-resolution imaging across large fields-of-view, often achieved by computational imaging approaches. Previously, we demonstrated 2D multimodal high-content microscopy via structured illumination microscopy (SIM) with resolution > 2x the diffraction limit, using speckle illumination from Scotch tape. In this work, we extend the method to 3D by leveraging the fact that the speckle illumination is in fact a 3D structured pattern. We use both a coherent and an incoherent imaging model to develop algorithms for joint retrieval of the 3D super-resolved fluorescent and complex-held distributions of the sample. Our reconstructed images resolve features beyond the physical diffraction-limit set by the system's objective and demonstrate 3D multimodal imaging with similar to 0.6 x 0.6 x 6 mu m(3) resolution over a volume of similar to 314 x 500 x 24 mu m(3). (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据