期刊
NATURE COMMUNICATIONS
卷 10, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-019-09985-2
关键词
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资金
- National Institutes of Health (NIH) [R01-GM110089, R21-EB021453]
- Medical Research Award
- W.M. Keck Foundation
- National Science Foundation (NSF GRFP)
- US National Institutes of Health K99/R00 Pathway to Independence Award from the National Institute of General Medical Sciences (NIGMS) [K99GM118909]
- Post 9/11 GI Bill
The CRISPR-Cas9 system provides the ability to edit, repress, activate, or mark any gene (or DNA element) by pairing of a programmable single guide RNA (sgRNA) with a complementary sequence on the DNA target. Here we present a new method for small-molecule control of CRISPR-Cas9 function through insertion of RNA aptamers into the sgRNA. We show that CRISPR-Cas9-based gene repression (CRISPRi) can be either activated or deactivated in a dose-dependent fashion over a >10-fold dynamic range in response to two different small-molecule ligands. Since our system acts directly on each target-specific sgRNA, it enables new applications that require differential and opposing temporal control of multiple genes.
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