期刊
NATURE COMMUNICATIONS
卷 10, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-019-10513-5
关键词
-
资金
- European Research Council (ERC) [616441 DISEASEAVATARS, 670821 PROTEOMICS4D]
- SystemcX.ch project PhosphoNet PPM
- Swiss National Science Foundation (SNSF) [31003A_166435]
- SystemsX [RTD 2012/191, 2013/156]
- PrECISE project from the European Union Horizon 2020 research and innovation programme [668858]
- SystemsX.ch project (PhosphoNet PPM) by Foundation for Research in Science and the Humanities at the University of Zurich (SWF)
- Westlake Startup Grant, Zhejiang Provincial Natural Science Foundation of China [LR19C050001]
Deterioration of biomolecules in clinical tissues is an inevitable pre-analytical process, which affects molecular measurements and thus potentially confounds conclusions from cohort analyses. Here, we investigate the degradation of mRNA and protein in 68 pairs of adjacent prostate tissue samples using RNA-Seq and SWATH mass spectrometry, respectively. To objectively quantify the extent of protein degradation, we develop a numerical score, the Proteome Integrity Number (PIN), that faithfully measures the degree of protein degradation. Our results indicate that protein degradation only affects 5.9% of the samples tested and shows negligible correlation with mRNA degradation in the adjacent samples. These findings are confirmed by independent analyses on additional clinical sample cohorts and across different mass spectrometric methods. Overall, the data show that the majority of samples tested are not compromised by protein degradation, and establish the PIN score as a generic and accurate indicator of sample quality for proteomic analyses.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据