期刊
FRONTIERS IN CELLULAR NEUROSCIENCE
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fncel.2019.00217
关键词
GABAergic synapse; GABAergic synapse development; GABA(A) receptor; cell autonomous; glutamate receptor; inhibitory synapse; excitatory synapse
资金
- NINDS Intramural Research Program
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [ZIANS003136] Funding Source: NIH RePORTER
In the adult brain GABA(A) receptors (GABA(A)Rs) mediate the majority of synaptic inhibition that provides inhibitory balance to excitatory drive and controls neuronal output. In the immature brain GABA(A)R signaling is critical for neuronal development. However, the cell-autonomous role of GABA(A)Rs in synapse development remains largely unknown. We have employed the CRISPR-CAS9 technology to genetically eliminate GABA(A)Rs in individual hippocampal neurons and examined GABAergic and glutamatergic synapses. We found that development of GABAergic synapses, but not glutamatergic synapses, critically depends on GABA(A)Rs. By combining different genetic approaches, we have also removed GABA(A)Rs and two ionotropic glutamate receptors, AMPA receptors (AMPARs) and NMDA receptors (NMDARs), in single neurons and discovered a striking dichotomy. Indeed, while development of glutamatergic synapses and spines does not require signaling mediated by these receptors, inhibitory synapse formation is crucially dependent on them. Our data reveal a critical cell-autonomous role of GABA(A)Rs in inhibitory synaptogenesis and demonstrate distinct molecular mechanisms for development of inhibitory and excitatory synapses.
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