期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 602, 期 -, 页码 21-31出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2016.03.021
关键词
Macromolecular crystallography; Synchrotron radiation; Beamline instrumentation; X-ray diffraction; Review; Experimental methods
资金
- NIGMS NIH HHS [P41 GM111244] Funding Source: Medline
Following pioneering work 40 years ago, synchrotron beamlines dedicated to macromolecular crystallography (MX) have improved in almost every aspect as instrumentation has evolved. Beam sizes and crystal dimensions are now on the single micron scale while data can be collected from proteins with molecular weights over 10 MDa and from crystals with unit cell dimensions over 1000 A. Furthermore it is possible to collect a complete data set in seconds, and obtain the resulting structure in minutes. The impact of MX synchrotron beamlines and their evolution is reflected in their scientific output, and MX is now the method of choice for a variety of aims from ligand binding to structure determination of membrane proteins, viruses and ribosomes, resulting in a much deeper understanding of the machinery of life. A main driving force of beamline evolution have been advances in almost every aspect of the instrumentation comprising a synchrotron beamline. In this review we aim to provide an overview of the current status of instrumentation at modern MX experiments. The most critical optical components are discussed, as are aspects of endstation design, sample delivery, visualisation and positioning, the sample environment, beam shaping, detectors and data acquisition and processing. (C) 2016 Published by Elsevier Inc.
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