期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 606, 期 -, 页码 134-142出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2016.07.020
关键词
BSA; Hydrogels; beta-aggregates; FTIR; Mechanical spectra; Cell-scaffold
资金
- Italian Ministry of University, and Research [B79E13000270005]
Aggregation and gelation of globular proteins can be an advantage to generate new forms of nanoscale biomaterials based on the fibrillar architecture. Here, we report results obtained by exploiting the proteins' natural tendency to self-organize in 3D network, for the production of new material based on BSA for medical application. In particular, at five different pH values the conformational and structural changes of the BSA during all the steps of the thermal aggregation and gelation have been analyzed by FTIR spectroscopy. The macroscopic mechanical properties of these hydrogels have been obtained by rheological measurements. The microscopic structure of the gels have been studied by AFM and SEM images to have a picture of their different spatial arrangement. Finally, the use of the BSA hydrogels as scaffold has been tested in two different cell cultures. (C) 2016 Elsevier Inc. All rights reserved.
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