期刊
TOXICOLOGY
卷 423, 期 -, 页码 105-111出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2019.05.016
关键词
Doxorubicin; L-arginine; Vascular toxicity; Reactive oxygen species; Apoptosis
资金
- Talent Foundation of the First Affiliated Hospital of Dalian Medical University [2017D044]
- Chang Jiang Scholar Program [T2017124]
- National Natural Science Foundation of China [81570391]
Background/aims: Patients with doxorubicin (Dox) treatment have a high risk of developing vascular toxicity with an unknown mechanism. L-arginine is a substrate for nitric oxide (NO). The decreased level of arginine-NO metabolite in Dox-treated cancer patients was associated with increased level of vascular damage, which promoted us to investigate the mechanism of Dox-induced vascular dysfunction and verify whether L-arginine supplement could alleviate this vasculotoxic effect. Method: Within a mouse model of Dox injection (5 mg/kg i.p., 2 or 4 weeks), we measured vascular relaxation, blood pressure, vascular NO generation, apoptosis, and oxidative stress. We tested the efficacy of L-arginine (1.5 mg/g/day, 4 weeks) on Dox-induced vascular relaxation, blood pressure, vascular NO generation, apoptosis, as well as oxidative stress. Results: Dox induced endothelium-dependent vascular dysfunction, which was associated with increased reactive oxidative stress (ROS) production and reduced NO generation in the vessel. ROS was required for Dox-induced apoptosis of both smooth muscle cells and endothelial cells. Dox treatment in mice increased blood pressure, but had no effect on vascular inflammation and fibrosis. L-aringine restored Dox-induced vascular dysfunction via enhancing vascular NO production and alleviating ROS-mediated apoptosis. Conclusion: We for the first time demonstrated L-arginine was effectively in suppressing Dox-induced vascular dysfunction, by attenuating vascular NO release and apoptosis. Our results provide a therapeutic target or a circulating marker for assessing vascular dysfunction which response to Dox treatment, and advance our understanding of the mechanisms of Dox-induced vascular dysfunction.
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